Reduced Representation Bisulfite Sequencing (RRBS)

How it works

RRBS uses restriction digestion and size selection to enrich CpG-rich DNA fragments before bisulfite conversion and sequencing. It provides single-base methylation measurements within the covered regions, making it well suited to comparisons of promoters, CpG islands and other enriched loci across samples.

Applications

• Study DNA methylation during cell differentiation and development
• Compare methylation in CpG-rich regions associated with disease or treatment
• Assess changes in DNA methylation following drug treatment

Key features

• Single-base resolution within sequenced regions
• Enrichment of CpG-rich regions makes efficient use of sequencing data
• Comparisons based on sites with adequate coverage across samples

Workflow

1. 01

   Extract DNA and perform restriction digestion

2. 02

   Select DNA fragments and perform bisulfite conversion

3. 03

   Amplify, assess and sequence libraries

4. 04

   Quantify methylation and compare covered sites and regions

We tailor the experimental workflow and analysis to your samples and study objectives.