ATAC-seq

How it works

ATAC-seq uses Tn5 transposase to fragment accessible chromatin and insert sequencing adapters, producing a genome-wide map of open regions. Comparing cells, tissues or treatments can reveal changes in accessibility; integration with RNA-seq and ChIP-seq helps connect these changes to gene regulation.

Applications

• Compare accessible chromatin between treatment and control groups
• Identify regulatory regions for follow-up and analyze enriched motifs
• Relate accessibility changes to gene expression and develop regulatory hypotheses

Key features

• A streamlined workflow, with low-input options assessed for the sample type
• Genome-wide profiling of chromatin accessibility
• Supports integration with transcriptomic and protein-DNA interaction data

Workflow

1. 01

   Prepare cells or nuclei and assess sample quality

2. 02

   Perform Tn5 tagmentation to insert sequencing adapters

3. 03

   Amplify, assess and sequence libraries

4. 04

   Identify accessible regions and analyze differential accessibility and regulatory features

We tailor the experimental workflow and analysis to your samples and study objectives.