How it works
CUT&Tag uses an antibody to recruit a protein A/G-Tn5 fusion enzyme to chromatin associated with the target protein. The enzyme fragments nearby DNA and inserts sequencing adapters, followed by library amplification. Applications include histone modification profiling and studies of RNA polymerase or other targets for which the assay is suitable.
Applications
- Map the genomic distribution of histone modifications
- Investigate RNA polymerase occupancy and other compatible targets
- Identify potential regulatory target genes through bioinformatics analysis
Key features
- Streamlines the workflow by avoiding conventional immunoprecipitation and sonication steps
- Low-input options for limited or precious samples
- Antibody and target compatibility evaluated during project design
Workflow
- 01
Prepare cells or nuclei and assess antibody suitability
- 02
Recruit pA/pG-Tn5 to antibody-bound targets
- 03
Perform in situ tagmentation and amplify libraries
- 04
Sequence libraries and assess signal quality and enriched regions
We tailor the experimental workflow and analysis to your samples and study objectives.