How it works
SMART-seq uses template switching during reverse transcription to generate and amplify cDNA from single cells or low-input RNA. Its full-length approach provides broader transcript coverage than end-tag methods, supporting expression, transcript structure and splicing studies.
Applications
- Profile gene expression in single cells and low-input RNA samples
- Investigate splice isoforms with sufficient transcript coverage
- Compare transcriptional changes during development and differentiation
Key features
- Designed for single cells and limited RNA inputs
- Full-length coverage supports transcript and splicing analysis
- Library strategy selected around RNA input and the transcripts of interest
Workflow
- 01
Assess single-cell or low-input RNA samples
- 02
Perform template-switching reverse transcription and amplify cDNA
- 03
Prepare and assess libraries, then sequence
- 04
Quantify expression and analyze transcript structure where supported by the data
We tailor the experimental workflow and analysis to your samples and study objectives.