RIP-seq

How it works

RIP-seq uses a target-specific antibody to isolate RNA-protein complexes. The recovered RNA is sequenced to identify associated transcripts and compare their enrichment. Antibody selection, input samples and controls are considered together during study design.

Applications

• Identify coding and noncoding RNAs associated with the target protein
• Compare RNA enrichment across experimental conditions
• Investigate the roles of RNA-binding proteins in post-transcriptional regulation

Key features

• Transcriptome-wide screening of protein-associated RNAs
• Supports analysis of mRNA, lncRNA and other RNA classes
• Complements CLIP-seq with an enrichment-based view of associated transcripts

Workflow

1. 01

   Assess the samples, target RNA-binding protein and antibody

2. 02

   Immunoprecipitate RNA-protein complexes

3. 03

   Purify RNA, prepare libraries and sequence

4. 04

   Identify enriched RNAs and analyze their functions

We tailor the experimental workflow and analysis to your samples and study objectives.