MeRIP-seq (m6A-seq)

How it works

MeRIP-seq uses m6A-specific antibodies to enrich modified RNA fragments, followed by sequencing and bioinformatics analysis. The resulting enrichment maps support comparisons across experimental conditions and studies linking RNA modifications to expression and phenotype.

Applications

• Investigate the functions of m6A writers, erasers and readers
• Compare m6A enrichment during disease, development or experimental treatment
• Integrate expression data to prioritize transcripts associated with modification changes

Key features

• Transcriptome-wide profiling of m6A enrichment
• Integration with input RNA data for expression-aware analysis
• Regional enrichment maps with resolution determined by assay design

Workflow

1. 01

   Prepare RNA and retain an input control

2. 02

   Fragment RNA and enrich m6A-modified fragments

3. 03

   Construct and sequence input and enriched libraries

4. 04

   Identify m6A-enriched regions and analyze their relationship with expression

We tailor the experimental workflow and analysis to your samples and study objectives.