Hi-C Sequencing

How it works

Hi-C captures chromatin contacts through crosslinking, digestion and proximity ligation, followed by sequencing to construct genome-wide interaction maps. These maps support comparisons of chromosome organization across conditions and integration with gene expression and epigenomic data.

Applications

• Compare chromatin contact patterns across experimental conditions
• Combine ChIP-seq with Hi-C to study histone modifications and genome organization
• Relate RNA-seq expression changes to features of the 3D genome

Key features

• Genome-wide profiling of spatial chromatin interactions
• Study design can include an in situ Hi-C workflow
• Sequencing depth and analysis selected for the required resolution and sample characteristics

Workflow

1. 01

   Assess samples and crosslink chromatin

2. 02

   Digest DNA, label fragments and perform proximity ligation

3. 03

   Enrich contact fragments, prepare libraries and sequence

4. 04

   Filter valid contacts and analyze contact matrices and genome organization

We tailor the experimental workflow and analysis to your samples and study objectives.