The Research Question
This client study investigates subcutaneous adipose hyperplasia in facial port-wine stains, asking how changes in cell populations relate to the underlying regulatory mechanisms.
Profiling Cells, Chromatin and Gene Expression
Single-cell analysis identifies populations such as adipose stem and progenitor cells. ATAC-seq and RNA-seq add complementary information about chromatin accessibility and gene expression.
Investigating the Regulatory Mechanism
The study examines the effects of SPSB1 on HDAC1 degradation and stability, with follow-up experiments that include ChIP-qPCR. Together, these approaches connect cellular changes with potential regulatory mechanisms.
Publication
SPSB1 Promotes Subcutaneous Adipose Hyperplasia in Facial Port-Wine Stains by Controlling HDAC1 Degradation and Stability Through Two Distinct Proteolytic Pathways
Advanced Science
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